[EasyTools] PCR Mixture Calculator

PCR Mixture Calculator

Sample Count:

Repeat Count:

For 10 pmol stock primer For 100 pmol stock primer

Calculation Result:

Typically, the cDNA concentration used in qPCR falls within the range of 10 ng/μL to 100 ng/μL. This range is commonly used in various experiments, but it may be adjusted based on the experiment's objectives and sample types.

This calculator is based on a cDNA volume of 1ul. PCR mixture 19 ul + cDNA 1 ul