[EasyTools] PCR Mixture Calculator

Easily calculate the required volumes of DW, Master Mix, and Primers for your PCR experiments. This tool automatically adjusts for pipetting errors based on sample count.

Sample Count (N):

Repeat Count (n):

Select Primer Concentration: 10 pmol stock 100 pmol stock

Calculation Result:

Note: Typically, the cDNA concentration used in qPCR falls within the range of 10 ng/μL to 100 ng/μL. This range is commonly used in various experiments but may be adjusted based on objectives and sample types.

* Calculation base: PCR mixture 19 μl + cDNA 1 μl (Total 20 μl reaction)