DNA Dilution Calculator
Enter DNA concentrations (ug/ul) for up to 10 samples and specify the final volume (ul) to calculate dilution volumes.
| Sample (ug/ul) | DNA (ul) | DW (ul) |
|---|
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Mouse Age Calculation - Excel and web calculator
This document explains two methods for calculating the age of a mouse. The first method involves using Excel to perform the calculations, while the second method utilizes an online web tool calculator for a more straightforward approach.
Mouse Age Calculation
Mouse Age Calculation in Excel
Follow these steps to calculate the age of a mouse in Excel:
Create a New Excel Document:
- Open Excel and create a new document.
Set Column Headers:
- In cell A1, type "Birthdate."
- In cell B1, type "Current Date."
- In cell C1, type "Age (Days)."
- In cell D1, type "Age (Weeks)."
Enter Dates:
- In cell A2, input the birthdate of the mouse (e.g., "2023-01-15").
- In cell B2, use the following formula to automatically input the current date:
=TODAY()
Calculate Age:
- In cell C2, enter the following formula:
=B2-A2 - In cell D2, use the following formula to convert age (days) into weeks (weeks old):
=INT(C2/7)
Check the Results:
- Cells C2 and D2 will display the current age of the mouse in days and weeks, respectively.
You can now calculate the age of a mouse in Excel using these steps. If you have any further questions or need assistance, feel free to ask.
Mouse Age Calculation in web calculator
How to Use
1. Enter the birthdate of the mouse in the input field above.
2. Click the "Calculate" button to determine the mouse's age.
3. The age of the mouse in days and weeks will be displayed below.
Enter the birthdate of the mouse:
[EasyTools] PCR Mixture Calculator
PCR Mixture Calculator
Calculation Result:
Typically, the cDNA concentration used in qPCR falls within the range of 10 ng/μL to 100 ng/μL. This range is commonly used in various experiments, but it may be adjusted based on the experiment's objectives and sample types.
This calculator is based on a cDNA volume of 1ul. PCR mixture 19 ul + cDNA 1 ul
Concentration Calculations Made Easy
New to chemistry and lab work? Understanding concentration measurements and solution units is essential for success. In this beginner's guide, we'll simplify these concepts, making them accessible and practical for your experiments. Join us as we explore the basics and equip you with the knowledge to excel in your scientific journey.
Concentration Calculations Made Easy
Molar Concentration (M)
: Molar concentration represents the number of moles (M) of solute present in 1 liter of solution. It is calculated as the ratio of the number of moles to the volume of the solution in liters.
"grams (g) = molecular weight × M (molar concentration) × L (volume)"
Example 1: If you want to prepare a 1 M NaCl solution with a volume of 1 liter, you can calculate the amount of NaCl needed as follows:
First, consider the molecular weight of NaCl, which is 58.44 g/mol.
Take into account the desired molar concentration, which is 1 M (1 mol/L).
The required amount of NaCl is calculated as follows:
Required NaCl (g) = Molecular Weight (g/mol) × Molar Concentration (mol/L) × Volume (L)
Required NaCl (g) = 58.44 g/mol × 1 mol/L × 1 L
Performing the calculation yields a required amount of 58.44 g of NaCl.
So, to prepare a 1 M NaCl solution with a volume of 1 liter, you would need 58.44 grams of NaCl.
EasyTools - Solution Dilution Calculator
Normality (N)
: Normality is the equivalent weight of a solute (in grams) per liter of solution. An equivalent is the amount of a substance that can either gain or lose one mole of electrons in a chemical reaction. It is used primarily in acid-base reactions.
"grams (g) = molecular weight / valence × N (normality) × L (volume)"
Example 1: If you want to prepare 1 L of a 1 N AgNO3 solution, the required amount of AgNO3 in grams can be calculated as follows:
Molecular weight of AgNO3 = 170
Valence = 1
Using the formula: grams (g) = molecular weight / valence × N (normality) × L (volume)
X g = 170/1 x 1 x 1 = 170 g
Therefore, by dissolving 170 grams of AgNO3 in 1 L of water, you'll obtain a 1 N AgNO3 solution.
When the valence is "1," the Molarity (M) and Normality (N) concentrations are the same.
Percentage Concentration
: Percentage concentration expresses the amount of solute as a percentage of the total solution weight or volume.
- Weight/Weight (% w/w): The weight of solute in grams per 100 grams of solution.
- Volume/Volume (% v/v): The volume of solute in milliliters per 100 milliliters of solution.
- Weight/Volume (% w/v): The weight of solute in grams per 100 milliliters of solution.
- Volume/Weight (% v/w): The volume of solute in milliliters per 100 grams of solution.
Parts Per Million (ppm)
: Parts per million is a unit for expressing very low concentrations. It represents the number of milligrams (mg) of solute per liter of solution.
Note: ppm stands for "parts per million," indicating one part in a million, or 1/1,000,000.
| Concentration Unit | Calculation Method | |
|---|---|---|
| Molar Concentration (M) | Moles of solute / Volume (liters) = mol/L | |
| Normality (N) | Equivalent weight of solute (g) / Volume (liters) = N | N = M * Equivalent Factor* |
| Percentage (% w/w) | Mass of solute (g) / Total mass of solution (g) x 100 = % w/w | |
| Percentage (% v/v) | Volume of solute (mL) / Total volume of solution (mL) x 100 = % v/v | |
| Percentage (% w/v) | Mass of solute (g) / Total volume of solution (mL) x 100 = % w/v | |
| Percentage (% v/w) | Volume of solute (mL) / Total mass of solution (g) x 100 = % v/w | |
| Parts Per Million (ppm) | Mass of solute (mg) / Total volume of solution (liters) = ppm | ppm = 1000 * M (mg/L) |
*Equivalent Factor (Normality - N)
: In chemistry, the Equivalent Factor, also known as Equivalent Weight, plays a vital role in normality (N) calculations. It represents the weight of a substance that can either gain or lose one mole of electrons or react with one mole of hydrogen ions (H⁺) in a chemical reaction.
Here's a simplified explanation of Equivalent Factors:
- For monoprotic acids and bases (e.g., HCl and NaOH), the Equivalent Factor is equal to the molar mass of the substance.
- For diprotic acids or bases (e.g., H2SO4), the Equivalent Factor is half the molar mass because one mole of the substance can neutralize two moles of H⁺ or OH⁻ ions.
- For polyprotic acids or bases, the Equivalent Factor is adjusted accordingly based on the reaction stoichiometry.
This concept is particularly valuable in normality (N) calculations, where it ensures that the concentration of substances in a solution is measured in equivalents per liter, accounting for their specific reactivity in chemical reactions. This knowledge is essential for accurate titrations and understanding the behavior of substances in various chemical processes.
[EasyTools] Protein Concentration Calculation
Protein Concentration Calculation from BCA assay
How to Use
1. Enter the concentration of each sample in the "Sample Concentrations (ug/mL)" section. You can leave some fields empty.
2. Input the common dilution factor and final sample volume.
3. Click the "Calculate Dilution" button to perform the calculation.
4. View the calculation results in the table. The sample with the lowest corrected concentration will be highlighted.
5. The required loading volumes for 50ug, 30ug, and 10ug of protein will be displayed.
Dilution Calculation Result:
| Sample | Original Concentration (ug/mL) |
Protein (ug/uL) |
Lysate (uL) |
Lysis Buffer (uL) |
|---|
Loading Volume (uL):
BCA Protein Assay Protocol
Pierce™ BCA Protein Assay Protocol
Materials and Equipment:
- Pierce™ BCA Protein Assay Kit (A55864)
- Sample (protein sample)
- BSA (Bovine Serum Albumin for creating a standard curve)
- Microplate (96 wells)
- Multi-pipette (for dispensing samples and standards)
- Spectrophotometer or plate reader
Steps:
1. Add your protein samples to each well of the microplate. Add 25-200 μg of protein to each sample well.
2. Dilute the standard solution to various concentrations, typically 0, 7.8, 15.6, 31.25, 62.5, 125, 250, 500 μg/mL, etc.
3. Prepare the Pierce™ BCA Reagent mixture: Mix 50 parts of Pierce™ BCA Reagent Solution A with 1 part of Pierce™ BCA Reagent Solution B. For example, mix 50 μL of Solution A with 1 μL of Solution B to create the working Pierce™ BCA Reagent solution. (A:B=50:1)
4. For each standard concentration and sample, add the Pierce™ BCA Reagent and mix well. Incubate the plate at 37°C for 30 minutes.
5. After the incubation, measure the absorbance at 562 nm using a spectrophotometer or plate reader.
6. Create a standard curve using the absorbance values for each standard. Plot the data and perform linear regression analysis.
7. Calculate the protein concentrations of your samples using the standard curve and the absorbance values obtained.
[EasyTools] Drug Dosage Calculator
Drug Dosage Calculator
Enter the following information and click the "Calculate" button to determine the drug volume to administer:
- Drug Concentration (mg/ml): Enter the concentration of the drug solution in milligrams per milliliter (mg/ml). For example, if it's 10 mg of drug in 1 ml of solution, enter 10 mg/ml.
- Mouse Weight (g): Enter the weight of the mouse in grams (g) that will receive the drug.
- Desired Dose (mg/kg): Enter the desired dose in milligrams per kilogram (mg/kg) of the mouse's body weight. For example, if you want to administer 20 mg of drug per kilogram of mouse body weight, enter 20 mg/kg.
Result: 0
[EasyTools] Solution Calculator
Solution Calculator
Enter the weight of the chemical, molecular weight, and the desired concentration to calculate the required solution volume.
[EasyTools] Solution Dilution Calculator
Solution Dilution Calculator
The solution dilution calculator calculates the volume of stock concentrate to add to achieve a specified volume and concentration. It uses the formula M1V1 = M2V2, where "1" represents the concentrated conditions (i.e., stock solution molarity and volume), and "2" represents the diluted conditions (i.e., desired volume and molarity).
[EasyTools] RNA concentration
RNA and DW Calculator
This simple calculator allows users to input RNA concentrations in ug/ul, and it calculates the amount of RNA needed for cDNA synthesis as well as the required volume of DW (distilled water). It can calculate the volume of reagents needed for cDNA synthesis and enables the download of both the entered RNA concentrations and the calculated RNA values.
Results:
| Sample | RNA Amount for cDNA (ul) | DW Volume (ul) |
|---|
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