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Showing posts with label proteins. Show all posts
Showing posts with label proteins. Show all posts

[EasyTools] Protein Concentration Calculation

Protein Concentration Calculation from BCA assay

Protein Concentration Calculation from BCA assay




How to Use

1. Enter the concentration of each sample in the "Sample Concentrations (ug/mL)" section. You can leave some fields empty.

2. Input the common dilution factor and final sample volume.

3. Click the "Calculate Dilution" button to perform the calculation.

4. View the calculation results in the table. The sample with the lowest corrected concentration will be highlighted.

5. The required loading volumes for 50ug, 30ug, and 10ug of protein will be displayed.



























4x Loading Buffer: 0 uL


Dilution Calculation Result:

Sample Original Concentration
(ug/mL)
Protein
(ug/uL)
Lysate
(uL)
Lysis Buffer
(uL)


Loading Volume (uL):