[EasyTools] Protein Concentration Calculation

Protein Concentration Calculator (BCA Assay)

This tool simplifies Western Blot sample preparation. Input your BCA assay results to automatically calculate protein concentrations, lysate volumes, and required lysis buffer to normalize your samples.

How to Use

1. Enter the raw concentration (ug/mL) for each sample.
2. Input the Dilution Factor used in the assay and the desired Final Volume for normalization.
3. Click Calculate. The tool will highlight the sample with the lowest concentration (limiting factor).

Sample Concentrations (ug/mL)

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Sample 6

Sample 7

Sample 8

Sample 9

Sample 10

Common Dilution Factor

Final Sample Volume (uL)

Required 4x Loading Buffer: 0 uL

Normalization Table

Sample	Raw Conc. (ug/mL)	Protein (ug/uL)	Lysate (uL)	Lysis Buffer (uL)

Required Volume for Gel Loading

* Values in parentheses indicate the total volume including 4x Loading Buffer (1.25x).