🧪 Resuspension Calculator
Calculate the volume needed to resuspend your dry oligos to a desired concentration.
Calculate the volume needed to resuspend your dry oligos to a desired concentration.
Enter DNA concentrations (ug/ul) for up to 10 samples and specify the final volume (ul) to calculate dilution volumes.
| Sample (ug/ul) | DNA (ul) | DW (ul) |
|---|
Typically, the cDNA concentration used in qPCR falls within the range of 10 ng/μL to 100 ng/μL. This range is commonly used in various experiments, but it may be adjusted based on the experiment's objectives and sample types.
This calculator is based on a cDNA volume of 1ul. PCR mixture 19 ul + cDNA 1 ul
1. Enter the concentration of each sample in the "Sample Concentrations (ug/mL)" section. You can leave some fields empty.
2. Input the common dilution factor and final sample volume.
3. Click the "Calculate Dilution" button to perform the calculation.
4. View the calculation results in the table. The sample with the lowest corrected concentration will be highlighted.
5. The required loading volumes for 50ug, 30ug, and 10ug of protein will be displayed.
| Sample | Original Concentration (ug/mL) |
Protein (ug/uL) |
Lysate (uL) |
Lysis Buffer (uL) |
|---|
Enter the following information and click the "Calculate" button to determine the drug volume to administer:
Result: 0
Enter the weight of the chemical, molecular weight, and the desired concentration to calculate the required solution volume.
The solution dilution calculator calculates the volume of stock concentrate to add to achieve a specified volume and concentration. It uses the formula M1V1 = M2V2, where "1" represents the concentrated conditions (i.e., stock solution molarity and volume), and "2" represents the diluted conditions (i.e., desired volume and molarity).
This simple calculator allows users to input RNA concentrations in ug/ul, and it calculates the amount of RNA needed for cDNA synthesis as well as the required volume of DW (distilled water). It can calculate the volume of reagents needed for cDNA synthesis and enables the download of both the entered RNA concentrations and the calculated RNA values.
| Sample | RNA Amount for cDNA (ul) | DW Volume (ul) |
|---|
Description: The "Complementary DNA Sequence Generator" is a web-based tool that allows users to generate the complementary DNA (cDNA) sequence of a given DNA sequence. It is a simple and user-friendly utility designed to assist in molecular biology and genetics research. Users can follow these steps to utilize the tool:
Usage Instructions:
Complementary Sequence:
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