[EasyTools] Interactive Western Blot Antibody Layout Tool

1. Select Antibodies

Select Category Select Antibody

Selected List

3. Layout Simulation

Number of Membranes:

Please select antibodies and click 'Calculate Layout'.

[EasyTools] Oligo Resuspension Calculator

🧪 Resuspension Calculator

Calculate the volume needed to resuspend your dry oligos to a desired concentration.

Oligonucleotide Quantity

nmol µg (OD based)

Desired Final Concentration

µM (pmol/µL) mM

Molecular Weight *Required for µg unit

Required Solvent Volume

0 µL

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[EasyTools] DNA Dilution Calculator

DNA Normalization Calculator

Final Volume (µL):

Dilution Ratio (for Lowest): No Dilution (1/1) 1/2 (x2) 1/5 (x5) 1/10 (x10) 1/20 (x20) 1/50 (x50)

💡 Logic: Automatically detects the sample with the lowest concentration.
That sample is diluted by the selected ratio (e.g., 1/5). All other samples are diluted more to match that exact target concentration.

Results

Target Concentration:
(Based on lowest sample: )

Sample	Initial (ng/µL)	DNA (µL)	DW (µL)	Total (µL)

[EasyTools] PCR Mixture Calculator

Easily calculate the required volumes of DW, Master Mix, and Primers for your PCR experiments. This tool automatically adjusts for pipetting errors based on sample count.

Sample Count (N):

Repeat Count (n):

Select Primer Concentration: 10 pmol stock 100 pmol stock

Calculation Result:

Note: Typically, the cDNA concentration used in qPCR falls within the range of 10 ng/μL to 100 ng/μL. This range is commonly used in various experiments but may be adjusted based on objectives and sample types.

* Calculation base: PCR mixture 19 μl + cDNA 1 μl (Total 20 μl reaction)

[EasyTools] Protein Concentration Calculation

Protein Concentration Calculator (BCA Assay)

This tool simplifies Western Blot sample preparation. Input your BCA assay results to automatically calculate protein concentrations, lysate volumes, and required lysis buffer to normalize your samples.

How to Use

1. Enter the raw concentration (ug/mL) for each sample.
2. Input the Dilution Factor used in the assay and the desired Final Volume for normalization.
3. Click Calculate. The tool will highlight the sample with the lowest concentration (limiting factor).

Sample Concentrations (ug/mL)

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Sample 6

Sample 7

Sample 8

Sample 9

Sample 10

Common Dilution Factor

Final Sample Volume (uL)

Required 4x Loading Buffer: 0 uL

Normalization Table

Sample	Raw Conc. (ug/mL)	Protein (ug/uL)	Lysate (uL)	Lysis Buffer (uL)

Required Volume for Gel Loading

* Values in parentheses indicate the total volume including 4x Loading Buffer (1.25x).

[EasyTools] Drug Dosage Calculator

Drug Dosage Calculator

Enter the following information and click the "Calculate" button to determine the drug volume to administer:

• Drug Concentration (mg/ml): Enter the concentration of the drug solution in milligrams per milliliter (mg/ml). For example, if it's 10 mg of drug in 1 ml of solution, enter 10 mg/ml.
• Mouse Weight (g): Enter the weight of the mouse in grams (g) that will receive the drug.
• Desired Dose (mg/kg): Enter the desired dose in milligrams per kilogram (mg/kg) of the mouse's body weight. For example, if you want to administer 20 mg of drug per kilogram of mouse body weight, enter 20 mg/kg.

Drug Concentration (mg/ml):

Mouse Weight (g):

Desired Dose (mg/kg):

Result: 0

[EasyTools] Solution Calculator

Solution Calculator

Enter the weight of the chemical, molecular weight, and the desired concentration to calculate the required solution volume.

Desired Concentration: M mM uM
Sample Amount (mg):
Molecular Weight (MW):

[EasyTools] Solution Dilution Calculator

Solution Dilution Calculator

The solution dilution calculator calculates the volume of stock concentrate to add to achieve a specified volume and concentration. It uses the formula M1V1 = M2V2, where "1" represents the concentrated conditions (i.e., stock solution molarity and volume), and "2" represents the diluted conditions (i.e., desired volume and molarity).

Stock concentration: M mM uM nM
Desired final volume: L mL uL
Desired concentration: M mM uM nM

[EasyTools] RNA concentration

[EasyTools] RNA & cDNA Synthesis Calculator

How to Use:
1. Set your Experimental Condition and Target RNA Amount.
2. Add samples and input concentrations (µg/µL).
3. Click Calculate to get RNA/DW volumes and Master Mix.
4. Negative DW values will be highlighted in red.

Experimental Condition:

Target RNA Amount (µg):

Input RNA Concentration (µg/µL)

Calculation Results

Sample ID	Measured (µg/µL)	RNA Vol (µL)	DW Vol (µL)

[EasyTools] Complementary DNA Sequence Generator

Reverse Complement DNA Generator

This web-based tool generates the Reverse Complement sequence of a given DNA strand. Essential for primer design, probe generation, and sequence analysis in molecular biology. It converts 5'-3' sequences into their anti-parallel complementary strands (5'-3').

How to Use:

1. Paste your DNA sequence (e.g., ATGC...) into the input box.
2. Numbers and whitespace will be automatically removed.
3. Click Generate to see the result.

Input Sequence (5' → 3'):

Length: 0 bp

Output Sequence (5' → 3'):